User: Yan Martel-Kennes (Sollio Agriculture)
This study investigates the effects of increasing doses of vitamin D3 supplementation on immune responses and performance in dairy cows facing immune challenges. While current practices regarding vitamin D3 appear adequate in normal conditions, the specific needs of animals for this nutrient during immune challenges are not well-defined. Eight Holstein cows will receive varying levels of dietary vitamin D3 supplementation. An inflammatory challenge will be induced using lipopolysaccharide (LPS) infusion to simulate immune activation safely and without the use of pathogens. The study aims to evaluate how vitamin D3 impacts key indicators of immune responses, including oxylipid concentrations, gene expression in immune cells, and milk production and composition. We hypothesize that vitamin D3 dosage will influence immune responses, allowing us to identify the optimal level of supplementation. The results of this study aim to provide insights into the optimal use of vitamin D3 during immune challenges, potentially improving overall health and performance in dairy farming.