{"id":33358,"date":"2025-11-10T17:50:24","date_gmt":"2025-11-10T22:50:24","guid":{"rendered":"https:\/\/genomequebec.wp.vortexdev.com\/en\/technological-services\/genotypage-par-sequencage-de-microsatellites\/"},"modified":"2025-11-11T17:36:30","modified_gmt":"2025-11-11T22:36:30","slug":"genotyping-by-microsatellite-sequencing","status":"publish","type":"service","link":"https:\/\/genomequebec.wp.vortexdev.com\/en\/technological-services\/genotyping-by-microsatellite-sequencing\/","title":{"rendered":"Genotyping by microsatellite sequencing"},"content":{"rendered":"\n<h2 class=\"wp-block-heading has-text-align-center\" id=\"top-of-page\"><strong><strong>3730xl DNA Analyzer Applied Biosystems Technology<\/strong><\/strong><\/h2>\n\n\n\n<h3 class=\"wp-block-heading has-text-align-center\"><em><strong><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-primary-color\">User Guide<\/mark><\/strong><\/em><\/h3>\n\n\n\n<p>The instructions in this guide must be followed carefully to achieve the best possible results and avoid delays in processing your request.<strong>\u202f<\/strong>&nbsp;<\/p>\n\n\n\n<p>Samples that do not comply with the recommendations may be rejected without compensation.<strong>\u202f<\/strong>&nbsp;<\/p>\n\n\n\n<p>Note that delays in the processing of samples will vary depending on the size of the project. It is recommended to contact the <a href=\"https:\/\/genomequebec.wp.vortexdev.com\/en\/contact-us\/\" target=\"_blank\" rel=\"noreferrer noopener\">Client Management Office<\/a> for more information.&nbsp;<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"#Starting-Material\" data-type=\"internal\" data-id=\"#Starting-Material\">Starting Material<\/a><\/li>\n\n\n\n<li><a href=\"#Requirements-Volume-and-concentration\" data-type=\"internal\" data-id=\"#Requirements-Volume-and-concentration\">Requirements \u2013 Volume and concentration<\/a><\/li>\n\n\n\n<li><a href=\"#Requirements-Quality\" data-type=\"internal\" data-id=\"#Requirements-Quality\">Requirements \u2013 Quality<\/a><\/li>\n\n\n\n<li><a href=\"#Solutions-Accepted-in-Empty-Wells\" data-type=\"internal\" data-id=\"#Solutions-Accepted-in-Empty-Wells\">Solutions Accepted in Empty Wells<\/a><\/li>\n\n\n\n<li><a href=\"#Required-containers-Sample-Organization-and-identification\" data-type=\"internal\" data-id=\"#Required-containers-Sample-Organization-and-identification\">Required containers \u2013 Sample Organization and identification<\/a><\/li>\n\n\n\n<li><a href=\"#Service-Request-Form-and-Sample-Submission\" data-type=\"internal\" data-id=\"#Service-Request-Form-and-Sample-Submission\">Service Request Form and Sample Submission<\/a><\/li>\n\n\n\n<li><a href=\"#Sample-Shipment-Preparation\" data-type=\"internal\" data-id=\"#Sample-Shipment-Preparation\">Sample Shipment Preparation<\/a><\/li>\n\n\n\n<li><a href=\"#Transmission-of-results\" data-type=\"internal\" data-id=\"#Transmission-of-results\">Transmission of results<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-f1ea74b888da7196577b0d8ad8e41b53\" id=\"Starting-Material\">Starting Material<\/h4>\n\n\n\n<p>The starting material for the microsatellite marker genotyping service may vary depending on the options chosen.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th class=\"has-text-align-center\" data-align=\"center\">Option<\/th><th class=\"has-text-align-center\" data-align=\"center\">Description<\/th><th class=\"has-text-align-center\" data-align=\"center\">Starting Material<\/th><\/tr><\/thead><tbody><tr><td class=\"has-text-align-center\" data-align=\"center\">1 <br>Read only<\/td><td class=\"has-text-align-left\" data-align=\"left\">Reading of PCR products previously resuspended in formamide with a standard molecular weight scale.<\/td><td class=\"has-text-align-center\" data-align=\"center\">PCR product* previously resuspended in formamide + standard<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">2 <br>Addition of formamide with standard + reading<\/td><td class=\"has-text-align-left\" data-align=\"left\">Reading of PCR products to be resuspended in formamide with the standard 500Liz molecular weight scale.<\/td><td class=\"has-text-align-center\" data-align=\"center\">PCR product*<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">3 <br>Addition of formamide with standard + reading + analysis<\/td><td class=\"has-text-align-left\" data-align=\"left\">Reading of PCR products to be resuspended in formamide with the standard 500Liz molecular weight scale and analysis of chromatograms.<\/td><td class=\"has-text-align-center\" data-align=\"center\">PCR product*<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">4 <br>Reading + analysis<\/td><td class=\"has-text-align-left\" data-align=\"left\">Reading of PCR products previously resuspended in formamide with a standard molecular weight scale and analysis of chromatograms.<\/td><td class=\"has-text-align-center\" data-align=\"center\">PCR product* previously resuspended in formamide + standard<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">5 <br>PCR + reading + analysis (10 markers)<\/td><td class=\"has-text-align-left\" data-align=\"left\">Carrying out PCR amplification from genomic DNA (gDNA), reading and analysis of the 10&nbsp;markers.**<\/td><td class=\"has-text-align-center\" data-align=\"center\">Genomic DNA<br>(human only)<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">6 <br>PCR + reading + analysis (24 markers)<\/td><td class=\"has-text-align-left\" data-align=\"left\">Carrying out PCR amplification from gDNA, reading and analysis of the 24 markers.**<\/td><td class=\"has-text-align-center\" data-align=\"center\">Genomic DNA <br>(human only)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>* All PCR products to be analyzed must have been amplified with a pair of primers, one of which is labeled with a fluorochrome. Accepted fluorochromes are FAM, VIC, NED and PET.<\/p>\n\n\n\n<p>**For the identification of human cell lines, the kits used include the following markers:<\/p>\n\n\n\n<p>GenePrint\u00ae 10: AMEL, CSF1PO, D13S317, D16S539, D21S11, D5S818, D7S820, TH01, TPOX, vWA.<\/p>\n\n\n\n<p>GenePrint\u00ae 24: Amelogenin, D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX D, YS391, D8S1179, D12S391, D19S433, FGA, D22S1045.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-b7e7efc4d888b20f923523cf4d73e0c5\" id=\"Requirements-Volume-and-concentration\">Requirements &#8211; Volume and concentration<\/h4>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th>Sample type<\/th><th>&nbsp; Option<\/th><th>&nbsp; Volume (concentration)<\/th><\/tr><\/thead><tbody><tr><td>PCR product in formamide<\/td><td>1 and 4<\/td><td>10 \u00b5L<\/td><\/tr><tr><td>PCR product*<\/td><td>2 and 3<\/td><td>10 \u00b5L<\/td><\/tr><tr><td>Genomic DNA (gDNA)<\/td><td>5 and 6<\/td><td>10 \u00b5L (10 \u00e0 50 ng\/\u00b5L)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>*For options 2 and 3, if the volume of 10 \u00b5L is not respected for each sample, the plate will be automatically rejected.<\/p>\n\n\n\n<p>It is the customer&#8217;s responsibility to provide samples of good quality and sufficient quantity.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-2391685e986c2c4d9ef4061608acc484\" id=\"Requirements-Quality\">Requirements \u2013 Quality<\/h4>\n\n\n\n<figure class=\"wp-block-table\"><table><thead><tr><th><strong><strong>Sample Type<\/strong><\/strong><\/th><th><strong><strong>Recommended method for verifying concentration<\/strong><\/strong><\/th><th><strong><strong>Recommended method for verifying purity<\/strong><\/strong><\/th><\/tr><\/thead><tbody><tr><td>gDNA<\/td><td>Qubit or equivalent fluorometric method.<br><br><strong>Note<\/strong>: Spectrophotometric methods such as Nanodrop are much less accurate for measuring DNA concentration.<\/td><td>Nanodrop or other spectrophotometric method.<br><br><strong>Target optical density ratios<\/strong>:<br><br>&#8211; 260\/280 between 1.8 and 2.0<br><br>&#8211; 260\/230 between 2.0 and 2.2<\/td><\/tr><tr><td>PCR Product<\/td><td colspan=\"2\">The concentration, size and integrity of the PCR products must have been verified by electrophoresis gel.<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-35fe26d7b29e1a82c314050c11eb424d\" id=\"Solutions-Accepted-in-Empty-Wells\">Solutions Accepted in Empty Wells<\/h4>\n\n\n\n<p>Each empty well must contain the same volume as a well that contains a PCR product by adding one of the solutions below:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Hi-Di\u2122 formamide<\/li>\n\n\n\n<li>EDTA 0.2 mM<\/li>\n\n\n\n<li>Ultra pure water type 1 (MilliQ water)<\/li>\n\n\n\n<li>UltraPure\u2122 DNase\/RNase-Free Distilled Water<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-35ac4714972000612540d06bfafd2885\" id=\"Required-containers-Sample-Organization-and-identification\">Required containers \u2013 Sample Organization and identification<\/h4>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th><strong><strong>Recommended tubes for gDNA<\/strong><\/strong><\/th><th><strong>96-well PCR plate recommended&nbsp;&nbsp;<\/strong><\/th><th><strong>96-well PCR plate unaccepted&nbsp;&nbsp;<\/strong><\/th><\/tr><\/thead><tbody><tr><td>&#8211; PCR strip tubes of 8 or 12<br>&nbsp;<br>All types of 200\u00b5L PCR tubes in strip of 8 or 12 with a cap are accepted.<br><br>Example: <em>0,2 mL PCR strip tubes with 12 well<\/em>, VWR, Cat # 53509-300&nbsp;<br><br>And <em>strip domed caps for 12 well strips<\/em>, VWR, Cat # 53509-302&nbsp;<\/td><td>&#8211; Half-skirt PCR plate<br>&nbsp;<br>All types of clear half-skirt 96-well PCR plates are accepted.<br><br>Example: <em>Thermo-Fast 96 PCR detection plate with flat deck<\/em>; Life Technologie, Cat# AB1400L<br><br>Example:<em> 96-well plate, standard semi-skirted, clear<\/em>; FroggaBio, Cat#SS-96S&nbsp;<\/td><td>&#8211; Cell culture plates<br><br>&#8211; No-skirt PCR plates<br><br>&#8211; Full-skirt PCR plates<br><br>&#8211; Opaques plates&nbsp;<\/td><\/tr><tr><td>&nbsp;<\/td><td>&#8211; Clear adhesive film<br>Example: Adhesive PCR Films; Thermo Fisher Scientific, Cat#AB0558<br>&nbsp;<br>&#8211; Aluminum adhesive film<br>Example : Adhesive PCR Foil Seals; VWR International, Cat#60941-074<br>&nbsp;<br>&#8211; Strip caps of 8 or 12 preventing any risk of leakage and cross-contamination.<br>&nbsp;<\/td><td>&nbsp;<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<ul class=\"wp-block-list\">\n<li>For the microsatellite marker genotyping service, 96-well plates can be submitted full.<\/li>\n\n\n\n<li>For options 1 and 4, read-only and for read-only + analysis, if a plate is not full, Type 1 ultrapure water (or another <span style=\"text-decoration: underline;\"><a href=\"#Solutions-Accepted-in-Empty-Wells\" data-type=\"internal\" data-id=\"#Solutions-Accepted-in-Empty-Wells\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-secondary-color\">accepted solution<\/mark><\/a><\/span>) must be added to each empty well.<\/li>\n\n\n\n<li>For the human cell line identification service, two wells per plate must be left empty for the addition of controls, wells H11 and H12.<\/li>\n\n\n\n<li>Samples must be placed in the plate as indicated on the submission form \u2013 <span style=\"text-decoration: underline;\">Row arrangement mandatory<\/span>.<\/li>\n<\/ul>\n\n\n\n<p>The name on the tube or plate must be unique,\u202freadable, concise, and identical to the name\u202findicated\u202fon the submission form.\u202f&nbsp;<\/p>\n\n\n\n<p>This is to avoid any delays in processing the request.<\/p>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-1afbfc3d09342a442ee7af6d6f5edfd7\" id=\"Service-Request-Form-and-Sample-Submission\">Service Request Form and Sample Submission<\/h4>\n\n\n\n<p>Click on&nbsp;<a href=\"https:\/\/genomequebec.wp.vortexdev.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/service-request-and-sample-submission\/\" target=\"_blank\" rel=\"noreferrer noopener\">service request and sample submission<\/a>&nbsp;to view instructions.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-096fea86e9448c1a40045488fbf1d398\" id=\"Sample-Shipment-Preparation\">Sample Shipment Preparation<\/h4>\n\n\n\n<p>Click on&nbsp;<a href=\"https:\/\/genomequebec.wp.vortexdev.com\/en\/technological-services\/centre-dexpertise-et-de-services-2\/instructions\/sample-shipment-preparation\/\" target=\"_blank\" rel=\"noreferrer noopener\">samples shipment preparation<\/a>&nbsp;to view instructions.<\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-secondary-color has-text-color has-link-color wp-elements-001b4d0493cf1fc677438e86372a3251\" id=\"Transmission-of-results\">Transmission of results<\/h4>\n\n\n\n<p>An email is sent as soon as the results are available.<\/p>\n\n\n\n<p>The reading and analysis results, if applicable, are directly accessible via the <a href=\"https:\/\/ces.genomequebec.com\/nanuqAdministration\/nanuq-administration\/welcomeHome.do\" target=\"_blank\" rel=\"noreferrer noopener\">Nanuq<\/a> web application.<\/p>\n\n\n\n<p>The raw data files generated by the 3730xl DNA Analyzer are in .fsa format.<\/p>\n\n\n\n<p><strong>Version 1.0_2025-11-06<\/strong><\/p>\n\n\n\n<div class=\"wp-block-buttons is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-03627597 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"#top-of-page\">Return to top of page<\/a><\/div>\n<\/div>\n","protected":false},"featured_media":0,"parent":0,"menu_order":0,"template":"","class_list":["post-33358","service","type-service","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/genomequebec.wp.vortexdev.com\/en\/wp-json\/wp\/v2\/service\/33358","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genomequebec.wp.vortexdev.com\/en\/wp-json\/wp\/v2\/service"}],"about":[{"href":"https:\/\/genomequebec.wp.vortexdev.com\/en\/wp-json\/wp\/v2\/types\/service"}],"wp:attachment":[{"href":"https:\/\/genomequebec.wp.vortexdev.com\/en\/wp-json\/wp\/v2\/media?parent=33358"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}