{"id":26809,"date":"2023-03-13T13:50:30","date_gmt":"2023-03-13T17:50:30","guid":{"rendered":"https:\/\/genomequebec.wp.vortexdev.com\/?post_type=educative-content&#038;p=26809"},"modified":"2026-01-16T10:07:58","modified_gmt":"2026-01-16T15:07:58","slug":"mission-edna","status":"publish","type":"educative-content","link":"https:\/\/genomequebec.wp.vortexdev.com\/en\/educative-content\/educational-space\/activities\/mission-edna\/","title":{"rendered":"Mission eDNA"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">What is environmental DNA?<\/h2>\n\n\n\n<p>We know that DNA is found in every cell of every living thing, so let&#8217;s imagine that a living thing loses a cell. What happens? A plant loses a leaf, a human being scratches himself and loses the superficial cells of his skin, an animal loses its hair, and so on. Living organisms constantly deposit genetic material in their environment.<\/p>\n\n\n\n<p>DNA can be released into the environment via faeces, urine, gametes, mucus, saliva and skin, and it can also come from the decomposition of dead organisms. In aquatic environments, dead, decomposing organisms release a phenomenal amount of genetic material.<\/p>\n\n\n\n<p>So, environmental DNA (eDNA) is the lost cells (intact or not) that we recover from the environment. Scientifically speaking, <strong>eDNA is genetic material derived from an environmental sample (freshwater, saltwater, sediment, humus, faeces, etc.).<\/strong><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-secondary-color\">Why is it useful?&nbsp;<\/mark><\/strong><\/h3>\n\n\n\n<p>In a context of climate change, it is more than imperative to have a good understanding of the ecosystems that surround us, so that we can better protect them. To implement appropriate strategies and actions to protect our ecosystems, we need to know which species inhabit them. Using sequencing, eDNA identification enables researchers to gain an overview of the community of living beings that inhabit a particular ecosystem, and to deduce trophic links and key species.<\/p>\n\n\n\n<p>Thanks to the eDNA we can extract from these water samples, we&#8217;ll have a better idea of the current state of biodiversity in the waterways around us, so we can better understand and protect them.<\/p>\n\n\n\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe title=\"(ENGLISH VERSION) MISSION ADN-eau: citizen science to the rescue of the environment.\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/5vGy0jBSo9s?list=PLLyndoVRYVtS6Ee05auqINQF3IUBxH-hd\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Mission eDNA : the project <\/strong><\/h2>\n\n\n\n<p>Qu\u00e9bec students, a major study of Qu\u00e9bec&#8217;s waterways is underway, and genomics researchers need your help! Your job is to bring back water samples containing the DNA of various species that live in these ecosystems!<\/p>\n\n\n\n<p>You&#8217;ll be working with a professional scientific team to use a powerful and very recent research tool: environmental DNA! It will enable you to recover the traces of DNA that living creatures (fish, invertebrates and microorganisms) leave behind in the water.<\/p>\n\n\n\n<p>With the help of the environmental DNA you&#8217;ve collected, we&#8217;ll be able to better understand the state of biodiversity in the rivers selected for the project.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>This project aims to:<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Contribute to a better understanding of the state of biodiversity in Quebec&#8217;s waterways.<\/li>\n\n\n\n<li>Gather valuable scientific data for the <a href=\"https:\/\/www.environnement.gouv.qc.ca\/index_en.asp\" data-type=\"URL\" data-id=\"https:\/\/www.environnement.gouv.qc.ca\/index_en.asp\" target=\"_blank\" rel=\"noopener\">minist\u00e8re de l\u2019Environnement, de la Lutte contre les changements climatiques, de la Faune et des Parcs<\/a><a href=\"https:\/\/www.environnement.gouv.qc.ca\/\" target=\"_blank\" rel=\"noopener\"> (gouv.qc.ca)<\/a>. This data can be used to implement the actions needed to conserve the biodiversity of the targeted watercourses.<\/li>\n\n\n\n<li>Open a dialogue between researchers from the various ministries involved and students to better understand the influence of human activity on the state of this biodiversity.<\/li>\n<\/ul>\n\n\n\n<p>The half-day activity will take place on the banks of a river. The results of the analyses will be revealed in the spring, during a live conversation between the students and the scientific team overseeing the project.<\/p>\n\n\n\n<p>This initiative was developed in collaboration with the minist\u00e8re de l\u2019Environnement, de la Lutte contre les changements climatiques, de la Faune et des Parcs and Universit\u00e9 Laval&#8217;s <a href=\"https:\/\/www.ibis.ulaval.ca\/en\/\" target=\"_blank\" rel=\"noreferrer noopener\">Institute of integrative biology and systems<\/a>. With financial support from the minist\u00e8re de l&#8217;\u00c9conomie et de l&#8217;Innovation.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"683\" src=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/DSC_7123-1024x683.jpg\" alt=\"\" class=\"wp-image-23286\" srcset=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/DSC_7123-1024x683.jpg 1024w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/DSC_7123-300x200.jpg 300w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/DSC_7123-768x512.jpg 768w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/DSC_7123-1536x1024.jpg 1536w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/DSC_7123.jpg 1920w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Related documents<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Here are all the documents you need to prepare and run the activity (French only):<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2024\/09\/1-GuideENSEIGNANT_2024-2.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Guide pour le personnel enseignant<\/a> (Teacher&#8217;s guide)<\/li>\n\n\n\n<li><a href=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2024\/08\/2.-Cahier-de-lELEVE_2024.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Cahier de l\u2019\u00e9l\u00e8ve<\/a> (Student workbook)<\/li>\n\n\n\n<li><a href=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2024\/08\/3.-Grille-dobservation_2024.pdf\" target=\"_blank\" data-type=\"link\" data-id=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2024\/03\/3.Grilledobservation_2021.pdf\" rel=\"noreferrer noopener\">Grille d\u2019observation<\/a> (Observation grid)<\/li>\n\n\n\n<li><a href=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2024\/08\/4.-Fiche-dhypothese-finale_2024.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Fiche hypoth\u00e8se<\/a> (Hypothesis sheet)<\/li>\n\n\n\n<li><a href=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2024\/08\/5.-Fiche-explicative-de-la-filtration-2024.pdf\" data-type=\"link\" data-id=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2024\/03\/5.Ficheexplicativedelafiltration_2021.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Fiche explicative de la filtration<\/a> (Filtration explanation sheet)<\/li>\n<\/ul>\n\n\n\n<h1 class=\"wp-block-heading\">How-to videos<\/h1>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe title=\"Mission ADN-eau : installation de la bouteille sur la perche\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/oduQX-voGT4?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe title=\"Mission ADN-eau : filtration\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/IR90X4flEuY?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe title=\"Mission ADN-eau : \u00c9tiquettes code \u00e0 barres et sac herm\u00e9tique\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/fUEPOV95xjE?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe title=\"\u00c0 go, c&#039;est Mission ADN-eau!\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/-9vy2skeQMQ?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div>\n<\/div>\n\n\n\n<h5 class=\"wp-block-heading\">Here are the three stages of the project (French only):<\/h5>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"1000\" height=\"231\" src=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/GQ-MISSION-ADN-5aout-e81tapes-site.jpg\" alt=\"\" class=\"wp-image-7343\" srcset=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/GQ-MISSION-ADN-5aout-e81tapes-site.jpg 1000w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/GQ-MISSION-ADN-5aout-e81tapes-site-300x69.jpg 300w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/GQ-MISSION-ADN-5aout-e81tapes-site-768x177.jpg 768w\" sizes=\"(max-width: 1000px) 100vw, 1000px\" \/><\/figure>\n\n\n\n<h4 class=\"wp-block-heading\"><strong>PHASE 1: Collecting data &#8211; Students<\/strong><\/h4>\n\n\n\n<p>This project couldn&#8217;t happen without your help! Your students will be the budding researchers who get this project off the ground. You&#8217;ll follow a precise research protocol established by a professional scientific team. This will enable you to collect and filter high-quality water samples, from which the eDNA can then be extracted.<\/p>\n\n\n\n<p><strong>You must ensure that your students follow the scientific protocol.<\/strong><\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><strong>PHASE 2: Analysis &#8211; The scientific team<\/strong><\/h4>\n\n\n\n<p><strong>Certifying the health of watercourses<\/strong><\/p>\n\n\n\n<p><strong>Once the species have been identified, researchers and students will get together to talk about the health of Quebec&#8217;s waterways.<\/strong><\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Experience overview<\/h4>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/GQ-schA9ma-phases-mA9thodologie.jpg\" alt=\"L\u2019attribut alt de cette image est vide, son nom de fichier est GQ-schA9ma-phases-mA9thodologie.jpg.\"\/><\/figure>\n\n\n\n<h4 class=\"wp-block-heading\"><strong>Methodology<\/strong><\/h4>\n\n\n\n<p><strong>A. Sampling:<\/strong> A water sample is taken from a stream. It contains traces of DNA from fish, invertebrates and microorganisms. This is eDNA.<\/p>\n\n\n\n<p><strong>B. Filtration: <\/strong>The water sample is passed through a fine filter by the students to recover the DNA fragments.<\/p>\n\n\n\n<p><strong>C. Hypothesis:<\/strong> Students are asked to formulate a hypothesis.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Phase 2 : Scientific team<\/h4>\n\n\n\n<p><strong>D. Extraction:<\/strong> The DNA is cleaned and preserved, while everything else is discarded (debris, dust, sediment, etc.).<\/p>\n\n\n\n<p><strong>E. Amplification:<\/strong> Our sample contains much more microbial DNA than fish and invertebrate DNA. As in the vast majority of ecosystems, the community of microorganisms in our river, though microscopic, is much larger than that of macroscopic species. The quantities of DNA collected will reflect this difference, and we can therefore expect our sample to contain 90% microbial DNA, compared with 10% DNA from other sources (fish, invertebrates, amphibians, humans, plants, etc.). In order to compensate for the possible biases this may entail during analysis, we amplify (copy in multiple copies) the DNA from fish and invertebrates, since these are the species we also want to study. Amplification takes place using a polymerase chain reaction (PCR). This technique, which takes place over several three-step cycles at different temperatures, produces millions of copies of a DNA molecule using the Taq DNA polymerase enzyme.<\/p>\n\n\n\n<p><strong>F. Sequencing:<\/strong> DNA fragments are read by a sequencer. The affinity of the base pairs is used to decode the order in which they are arranged. To do this, a single-stranded DNA fragment is deposited in a solution containing modified free nitrogenous bases. Luminescent molecules have previously been linked to the various nitrogenous bases. (e.g. Adenine &#8211; red, Cytosine &#8211; yellow, Thymine &#8211; green and Guanine &#8211; blue). The modified nitrogenous bases are left to form the DNA strand complementary to our fragment. The nitrogenous bases always associate with the same specificity: adenine with thymine (A-T or T-A) and cytosine with guanine (C-G or G-C). With the sequencer, we can read the &#8220;colored&#8221; sequence attached to our initial DNA fragment. For a given sequence: red-blue-green-yellow-green-blue-red-red, we can deduce that the sequence of luminescent nitrogenous bases is: A-G-T-C-T-G-A-A and that the initial DNA sequence was: T-C-A-G-A-C-T-T. Record these readings in a computer file.<\/p>\n\n\n\n<p><strong>G. Bioinformatics analysis: <\/strong>Public databases contain the complete genome sequence of the different species studied, so we can compare the sequence we have read with those recorded. So we search the databases of fish, invertebrates and microorganisms for the species to which the DNA we&#8217;ve collected and sequenced belongs.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Phase 3: Together<\/h4>\n\n\n\n<p>H. Results and interpretation: The researchers analyze the results and try to understand what they mean.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Why do we find more microorganisms in one river than another?<\/li>\n\n\n\n<li>Why isn&#8217;t such and such a species of fish found in this stream?<\/li>\n\n\n\n<li>What do these data say about the health of the streams we sampled?<\/li>\n<\/ul>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading\">The results map<\/h4>\n\n\n\n<figure class=\"wp-block-image size-large img-hover\"><a href=\"https:\/\/genomequebec.wp.vortexdev.com\/en\/interactive-map\/\" target=\"_blank\" rel=\" noreferrer noopener\"><img decoding=\"async\" width=\"1024\" height=\"678\" src=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/Explorer-la-carte-1-1024x678.png\" alt=\"\" class=\"wp-image-33558\" style=\"aspect-ratio:16\/9;object-fit:cover\" srcset=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/Explorer-la-carte-1-1024x678.png 1024w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/Explorer-la-carte-1-300x199.png 300w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/Explorer-la-carte-1-768x509.png 768w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/Explorer-la-carte-1.png 1357w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n\n\n\n<h4 class=\"wp-block-heading\">In collaboration with<\/h4>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><a href=\"https:\/\/www.hydroquebec.com\/\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" width=\"1024\" height=\"326\" src=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/logo-noir_HQ_600dpi-2-1024x326.jpg\" alt=\"\" class=\"wp-image-33662\" style=\"width:245px;height:auto\" srcset=\"https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/logo-noir_HQ_600dpi-2-1024x326.jpg 1024w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/logo-noir_HQ_600dpi-2-300x96.jpg 300w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/logo-noir_HQ_600dpi-2-768x245.jpg 768w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/logo-noir_HQ_600dpi-2-1536x489.jpg 1536w, https:\/\/genomequebec.wp.vortexdev.com\/wp-content\/uploads\/2023\/03\/logo-noir_HQ_600dpi-2-2048x652.jpg 2048w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n\n\n\n<p><\/p>\n","protected":false},"featured_media":7335,"parent":23747,"menu_order":0,"template":"","format":"standard","class_list":["post-26809","educative-content","type-educative-content","status-publish","format-standard","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/genomequebec.wp.vortexdev.com\/en\/wp-json\/wp\/v2\/educative-content\/26809","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/genomequebec.wp.vortexdev.com\/en\/wp-json\/wp\/v2\/educative-content"}],"about":[{"href":"https:\/\/genomequebec.wp.vortexdev.com\/en\/wp-json\/wp\/v2\/types\/educative-content"}],"up":[{"embeddable":true,"href":"https:\/\/genomequebec.wp.vortexdev.com\/en\/wp-json\/wp\/v2\/educative-content\/23747"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/genomequebec.wp.vortexdev.com\/en\/wp-json\/wp\/v2\/media\/7335"}],"wp:attachment":[{"href":"https:\/\/genomequebec.wp.vortexdev.com\/en\/wp-json\/wp\/v2\/media?parent=26809"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}